Introduction to 24h urinary protein quantification

The detection of 24-hour urinary protein quantitative test is to collect 24-hour urine, and use biuret method and dye binding method to detect it. The standard of judgment is that the normal value is 0.2-1.0g/24h,(+-(++) is usually 1-2g/24h,(+++-(++++) is usually greater than 3g/24h. Proteinuria is caused by damage to the glomerular filtration membrane, resulting in a large amount of protein leakage, and 95% of the protein is reabsorbed in the proximal convoluted tubules. When the tubules are damaged, proteinuria appears because of reabsorption disorders. In addition, the plasma of relatively low molecular weight protein abnormal increase, through glomerular filtration, beyond the renal tubular reabsorption capacity and out of the urine, finally because of the distal convoluted tubule origin secretion of glycoprotein increased, resulting in increased urine protein.